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| Surenciski,Mauro Rodrigo; Flachsland,Eduardo Alberto; Terada,Graciela; Mroginski,Luis Amado; Rey,Hebe Yolanda. |
| The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30°C for 1 min, rehydrated using the same liquid mediums (0.75 M sucrose (24 h), 0.5 M... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryoconservation; Liquid nitrogen; Calcium alginate; Activated charcoal; Sucrose. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100002 |
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| Jin,Yuan-yuan; Li,Ya-dong; Sun,Wan; Fan,Shuai; Feng,Xiao-zhou; Wang,Kang-you; He,Wei-qing; Yang,Zhao-yong. |
| Background: D-Hydroxyphenylglycine is considered to be an important chiral molecular building-block of antibiotic reagents such as pesticides, and β-lactam antibiotics. The process of its production is catalyzed by D-hydantoinase and D-carbamoylase in a two-step enzyme reaction. How to enhance the catalytic potential of the two enzymes is valuable for industrial application. In this investigation, an Escherichia coli strain genetically engineered with D-hydantoinase was immobilized by calcium alginate with certain adjuncts to evaluate the optimal condition for the biosynthesis of D-carbamoyl-p-hydroxyphenylglycine (D-CpHPG), the compound further be converted to D-hydroxyphenylglycine (D-HPG) by carbamoylase. Results: The optimal medium to produce... |
| Tipo: Journal article |
Palavras-chave: Calcium alginate; D-Carbamoyl-p-hydroxyphenylglycine; D-Hydantoinase; D-Hydroxyphenylglycine; Immobilization; Whole cell. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300004 |
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